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Osteoarthritis is a common degenerative joint disease, and cartilage damage is often considered an early factor in irreversible joint degeneration. Repairing damaged cartilage remains a medical challenge due to its limited ability to self-repair and regenerate. In recent years, the application of tissue engineering strategies to treat cartilage defects has been recognized as an emerging therapeutic avenue. Acellular cartilage matrix (ACM) is an ideal material for cartilage repair and regeneration as it retains the extracellular matrix structure and bioactive components of natural cartilage, mimicking the extracellular environment of natural cartilage to the greatest extent. Type II collagen is the main type of hyaline cartilage and plays an important role in regulating the mechanical properties of cartilage tissue. It has been shown that type II collagen, growth factors and the hypoxic microenvironment play important roles in promoting cartilage regeneration. Type II collagen induces cell aggregation and chondrogenic differentiation in a specific way; Various growth factors contained in the ACM induce Sox9 expression and promote chondrogenic differentiation of stem cells; The hypoxic microenvironment upregulates the expression of type II collagen (COL2A1), Sox9 and maintains chondrocyte phenotype. In addition, ACM has been widely used in cartilage regeneration studies, either as a decellularized scaffold, hydrogel or 3D bioprinting technique for the repair of defective cartilage. Although the ACM-derived biomaterials discussed in this paper have many advantages, there are still some difficulties in their practical applications, such as loss of ACM components and reduced scaffold performance, which are still worth exploring in depth.
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Objective:To investigate the intention to obtain disease related information and its influencing factors among the elderly at their end-of-life stage.Methods:A cross-sectional survey was conducted among 414 elderly people aged ≥60 in 7 long-term residential care facilities and 15 communities in the central urban area of Fuzhou using the late-life care preference questionnaire from October 2016 to June 2017. Univariate analysis, multiple linear regression analysis and ordered multi-classification logistic regression analysis were used to identify information preference of the disease-related information and influencing factors among the elderly.Results:The level of disease-related information needs of the elderly was scored (17.1±4.9); 48.8% (202/414) preferred detailed or maximum information; 30.7% (127/414) wanted selective and partial information; 20.5% (85/414) did not want to know any information. Multiple linear regression analysis showed that age, education, and whether or not they had received or seen other life-sustaining treatments were the main factors affecting the level of disease-related information needs (standardized regression coefficients were -0.141, 0.116, 0.115, all P<0.05); ordered multi-classification logistic regression analysis showed that age (compared with 60-69 years old, 70-79 years old: OR=0.544, 95% CI: 0.310-0.957; 80-89 years old: OR=0.526, 95% CI: 0.289-0.956), education level (compared with primary school and below, college or above: OR=2.166, 95% CI: 1.093-4.290), main sources of living expenses (compared with other allowance, family support: OR=7.303, 95% CI: 1.157-46.108, pension: OR=9.288, 95% CI: 1.502-57.415; provident fund scheme/saving: OR=15.676, 95% CI: 2.122-115.793), whether or not they had received or seen other life-sustaining treatments (compared with yes, OR=1.985, 95% CI: 1.150-3.425) were the main factors affecting the level of disease-related information needs. Conclusions:Most of the elderly prefer to know disease-related information. Age, educational level, main sources of living expenses and whether or not they had received or seen other life-sustaining treatments are the main influencing factors.
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Serine incorporator (SERINC) is a multi-transmembrane family of carrier proteins that is highly conserved among species and helps to integrate serine into lipid bilayers to efficiently synthesize phospholipids Phosphatidylserine and sphingolipids. Recent studies have found that SERINC protein family, especially SERINC3 and SERINC5, act on many different viruses, such as human immunodeficiency virus (HIV) which causes AIDS, inhibit the replication and proliferation of the virus to achieve antiviral effects. The study on the mechanism of virus-host cell interaction has been a hot spot in the field and has important scientific significance. In this article, the structural features of SERINC protein family, the major biological functions, and the interaction with the virus in the anti-virus process are summarized in order to provide important information for the research on the host-virus interaction.
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OBJECTIVE:To establish quality standard of Fushiming capsule. METHODS:TLC was used to qualitatively identify the ligustilide,aurantio obtusin,chrysophanol,fruit of Chinese wolfberry and Whitmania pigra,respectively. HPLC method was used to determine the contents of puerarin and ginsenosides Rb1. The determination was performed on Intersil C18 column with mobile phase consisted of acetonitrile-0.3% phosphoric acid(gradient elution)at flow rate of 1.0 mL/min;the detection wavlength was set at 203 nm,and column temperature was 25 ℃;the sample size was 10 μL. RESULTS:TLC spots of ligustilide,aurantio obtusin,chrysophanol,the fruit of C. wolfberry and W. pigra were clear and well separated without negative interference. The linear range of puerarin and ginsenoside Rb1were 10.56-337.92 μg/mL(r=0.999 7)and 17.80-569.70 μg/mL(r=0.999 6). The limits of quantitation were 2.20,1.86 μg/mL,and the limits of detection were 0.12,0.13 μg/mL,respectively. RSDs of precision,stability and reproducibility tests were lower than 2.0%. The recoveries were 95.65%-99.66%(RSD=1.45%,n=6) and 96.95%-98.52%(RSD=0.77%,n=6),respectively. CONCLUSIONS:Established quality standard can be used for the quality control of Fushiming capsule.
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Objective To examine the effects of two different cold compress modes (continuous cold compress and intermittent cold compress) on the pain and swelling among patients receiving extraction of impacted tooth of mandibular. Methods From January 2016 to August 2016, 64 cases of adult patients with impacted mandibular impacted wisdom teeth and 24 h in the outpatient department of Oral and Maxillofacial Surgery of Affiliated Stomatological Hospital of Fujian Medical University were selected. They were randomly assigned to the control group and the experimental group (32 cases in each group) by random digital table. The participants in the control group received the usual care plus intermittent cold compress. The participants in the experimental group received the usual care plus continuous cold compress. And the treatment was continued for 24 hours. Outcomes including pain and skin temperature were measured in the intervention cycle, and the swelling was measured at baseline, 6 hour, 12 hours,18 hours, and 24 hours after the intervention. All the participants were asked to have further consultation at 7th days after the operation. Results At 6 hour, 12 hours,18 hours, and 24 hours, the pain of the experimental group was lower than the control group ,and there was significant difference (F=63.665-290.872, P<0.05 ). At 12 hours,18 hours, and 24 hours after the intervention ,number of the swelling grades (0-I-Ⅱ-III) in the control group was 16, 14, 2, 0 cases, 11, 13, 8, 0 cases, 11, 12, 8, 1 cases,number of the swelling grades (0-I-Ⅱ-III) in the experimental group was 27, 5, 0, 0 cases, 26, 6, 0, 0 cases, 26, 6, 0, 0 cases, and there was significant difference (Z=-2.968,-4.017,-4.052, P<0.05). A positive correlation between swelling grade and time (M2=45.22, P<0.05). At 6 hour, 12 hours,18 hours, and 24 hours , the skin temperature of the experimental group was lower than the control group, and there was significant difference (F=1 735.106-23 993.33, P<0.05). Conclusions This study showed that the continuous cold compress was more effective in reducing the pain and swelling among the patients receiving extraction of impacted tooth of mandibular. Compared with the intermittent cold compress, it suggested that continuous cold compress can be integrated into usual care to reduce the pain and swelling among the patients receiving extraction of impacted tooth of mandibular.
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Objective To establish a HPLC method for simultaneous determination of seven constituents in Pifubingxuedu tablet.Methods The determination was performed on Kromasil-C18 column (4.6 mm × 250 mm, 5 μm).The mobile phase was acetonitrile-0.5％ phosphoric acid aqueous solution with a flow rate of 0.8ml/min.The detector was PDA and detection wavelength was set at 245,325,403 nm, respectively.Results A method has been established for the determination of chlorogenic acid, hydroxy Safflower Pigment A, paeoniflorin, forsythiaside A, ferulic acid, berberine and glycyrrhizin acid in one run by HPLC.Their average contents and RSD in each Pifubingxuedu tablet were 0.299 5 mg/tablet (2.25％);0.700 0 mg/tablet(1.33％);0.429 2 mg/tablet (1.21％);0.039 1 mg/tablet (2.34％);0.014 8 mg/tablet(2.23％);0.209 0 mg/tablet (2.06％);0.272 7 mg/tablet (2.68％), respectively.Conclusion The established method is simple, convenient, accurate and reliable.It is suitable for the quality control of Pifubingxuedu tablet.
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Objective To verify the antiviral effects of Siji Kangbingdu mixture (SJKBDM) against coxsackievirus A16 (CoxA16).Methods Vero cells and 5-day-old suckling mice, injected with 75/50 and 1×106 TCID50 CoxA16, were used as evaluation models.The preventive influences of SJKBDM against CoxA16 in Vero cells were assessed in the models.The effects of SJKBDM on the mortality, survival time, change rate of body weight, and clinical symptom scores of suckling mice were observed.Results ①The half maximal inhibitory concentration of SJKBDM on Vero cells was 9.59 mg·mL-1.②Toxic effects were not observed from 32.3 g·kg-1 single dose or continuous intraperitoneal injectin of SJKBDM in suckling BALB/c mice.③The SJKBDM had significant inhibitory effect against CoxA16 virus.Doses higher than 1.22 mg·mL-1 could significantly improve the Vero cell survival rate, and the SJKBDM inhibition of 75/50 TCID50 CoxA16 induced pathological changes in Vero cells.④The SJKBDM significantly improved clinical symptoms of mice with CoxA16 viral infection, especially with crude drug doses of higher than 1.62 g·kg-1.The survival rate and other indicators were comparable or slightly higher compared with ribavirin, and the clinical score was higher than that of ribavirin.Conclusion The SJKBDM has significant inhibitory effect on CoxA16 cell proliferation, significantly decreases death rate, and improves clinical symptoms of mice infected with CoxA16 virus.